ANALYSIS OF CLOVE (Syzygium aromaticum L.) OIL OBTAINED WITH HYDRODISTILLATION AND ULTRASOUND- ASSISTED EXTRACTION

Abstract

Essential oils derived from various aromatic plants are frequently used due to their antioxidative, antibacterial and anti-inflammatory activity. Their pleasant odor makes them very useful in pharmaceutical, cosmetic and food industries. Clove oil (CO) can be isolated from dried flower buds of Syzygium aromaticum L. and its major component is eugenol presented in 70-90%. The aim of this study was to develop two extraction techniques and analyze the obtained CO with thin-layer chromatography (TLC). Clove buds purchased from the local market were powdered and added distilled water for hydrodistillation and 50% ethanol for ultrasound-assisted extraction. Liquid-liquid extraction was performed with dichloromethane and dried with anhydrous sodium sulfate. Accurate identification of eugenol with TLC was achieved with toluene:ethyl acetate (93:7) mixture as a mobile phase while high-efficiency separation of more active compounds was performed with ultrasound-assisted extraction technique.